![]() The major advance offered by NGS is the ability to produce an enormous volume of sequence data at an unprecedented speed and a constantly decreasing cost. In some respects, the potential of NGS is akin to the early days of PCR, with one's imagination being the primary limitation to its use. When do I use Sanger Sequencing vs.Next Generation Sequencing (NGS) has progressed enormously over the past ten years, transforming the biological sciences and opening up many new opportunities in basic, applied and clinical research. Next Generation Sequencing (NGS) – An Introduction These Youtube videos give a useful overview of how Sanger sequencing and the different next generation sequencing technologies work and when to use them: The next few pages provide step-by-step explanations of how each of these NGS technologies work.
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